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dc.contributor.authorKleven, Oddmund
dc.contributor.authorDawson, Deborah A.
dc.contributor.authorGjershaug, Jan Ove
dc.contributor.authorHorsburgh, Gavin J.
dc.contributor.authorJacobsen, Karl-Otto
dc.contributor.authorWabakken, Petter
dc.date.accessioned2019-03-29T09:41:35Z
dc.date.available2019-03-29T09:41:35Z
dc.date.created2013-03-21T13:43:48Z
dc.date.issued2013
dc.identifier.citationConservation Genetics Resources. 2013, 5 (3), 723-727.
dc.identifier.issn1877-7252
dc.identifier.urihttp://hdl.handle.net/11250/2592397
dc.description.abstractWe isolated 134 eagle-owl (Bubo bubo) microsatellite sequences. Eight of these newly isolated loci were characterized in 38 Eurasian eagle-owls in a northern European population. Sequence homology was used to assign a predicted chromosome location for the eight loci. We also redesigned primers for four previously isolated eagle-owl sequences and cross-amplified two published primer sets previously characterized in other owl species. These 14 loci were amplified in three multiplex PCR sets and displayed 2–9 alleles with expected and observed heterozygosities ranging from 0.34 to 0.85 and from 0.42 to 0.97, respectively. Estimated frequencies of null-alleles were low and only one locus deviated from Hardy– Weinberg equilibrium. After correcting for multiple tests, linkage disequilibrium was found for a single pair of loci. The combined probability of identity for the 14 loci was 3.5 9 10-12. These microsatellite loci are expected to be useful for genetic monitoring, parentage analysis and population genetic studies. bubo bubo, microsatellite loci, multiplex PCR, non-invasive samples, predicted genome locations
dc.language.isoeng
dc.titleIsolation, characterization and predicted genome locations of Eurasian eagle-owl (Bubo bubo) microsatellite loci
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionacceptedVersion
dc.source.pagenumber723-727
dc.source.volume5
dc.source.journalConservation Genetics Resources
dc.source.issue3
dc.identifier.doi10.1007/s12686-013-9891-y
dc.identifier.cristin1020713
dc.relation.projectNorges forskningsråd: 193818
cristin.unitcode209,98,55,2
cristin.unitnameInstitutt for skog- og utmarksfag
cristin.ispublishedtrue
cristin.fulltextpostprint
cristin.qualitycode1


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