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dc.contributor.authorJohansen, Wenche
dc.contributor.authorGrove, Søren
dc.contributor.authorAnonsen, Jan Haug
dc.contributor.authorMoen, Anders
dc.contributor.authorAgusti-Ridaura, Celia
dc.contributor.authorAzar, Amir Sefidmouy
dc.contributor.authorStrætkvern, Knut Olav
dc.date.accessioned2019-10-03T11:16:49Z
dc.date.available2019-10-03T11:16:49Z
dc.date.created2019-06-27T10:23:22Z
dc.date.issued2019
dc.identifier.issn0145-305X
dc.identifier.urihttp://hdl.handle.net/11250/2620060
dc.description.abstractComplement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates. We report the characterization of C5 cDNA and protein from Atlantic salmon (Salmo salar), a teleost fish species of high importance in aquaculture. The C5 cDNA cloned from liver is 5079 nucleotides long, whose translation product has a molecular weight of 190 kDa, with the classical β-α orientation and motifs/sites for β-α cleavage (678RPKR681) and cleavage by C5 convertases (R758). Mass spectrometric analysis show a single N-linked, biantennary, complex glycan at N1125. Moreover, the N-linked glycan displays an unusual modification in the form of acetylated sialic acid residues. Three anti-C5 antisera produced in mice using purified C5 worked in immunohistochemical analyses of formalin fixed liver tissue. The purification method, whereby inactive and activated (C5b) forms were isolated, opens for interesting studies on the complement function in fish, including possible connection to stress, disease and glycosylation.
dc.language.isoeng
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0145305X19300254?via%3Dihub
dc.titleComplement factor C5 in Atlantic salmon (Salmo salar): characterization of cDNA, protein and glycosylation
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
dc.source.volume100
dc.source.journalDevelopmental and Comparative Immunology
dc.identifier.doi10.1016/j.dci.2019.103424
dc.identifier.cristin1708211
cristin.unitcode209,2,2,0
cristin.unitnameInstitutt for bioteknologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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