Genetic admixture between Central European and Alpine wolf populations

Abstract

The recovery and expansion of formerly isolated wolf populations in Europe raise questions about the nature of their interactions and future consequences for population viability and conservation. Will fragmented populations fuse or maintain a certain level of isolation with migration? Central Europe is suitable for obtaining empirical data in this field as it represents a ‘crossroad' with the potential for contact among several phylogeographic lineages. In this study, non-invasive genetic samples obtained during population monitoring in the Bohemian and Bavarian Forest (BBF) mountain ranges in the Czech Republic and Germany (Bohemian Massif) were analysed at different neutral markers including mitochondrial sequence, nuclear autosomal microsatellites and gonosomal sex markers. Resultant genetic profiles were compared with reference data to study population ancestry. Both cluster analyses of microsatellite genotypes and syntopic occurrence of haplotypes HW01 and HW22 showed genetic admixture between Central European and Alpine populations. This represents secondary contact and interbreeding of formerly allopatric populations with different phylogeographic histories and distant expansion centres in different biomes in the Baltic region versus the Apennine peninsula and Alps. Moreover, the study describes the founding event and genealogy of this admixed deme, inhabiting intermediate environmental conditions compared to parental forms, and emphasises the role of protected areas as stepping stones in the range recolonization process in endangered large mammals.