Blar i Brage INN på forfatter "Demko, Viktor"
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An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing.
Ako, Ako Eugene; Perroud, Pierre-François; Innocent, Joseph; Demko, Viktor; Olsen, Odd-Arne; Johansen, Wenche (Journal article; Peer reviewed, 2017)Gene targeting is a powerful reverse genetics technique for site-specific genome modification. Intrinsic homologous recombination in the moss Physcomitrella patens permits highly effective gene targeting, a characteristic ... -
Membrane-anchored calpains – hidden regulators of growth and development beyond plants?
Safranek, Martin; Shumbusho, Alain; Johansen, Wenche; Sarkanova, Julia; Vosko, Stanislav; Bokor, Boris; Jasik, Jan; Demko, Viktor (Peer reviewed; Journal article, 2023)Calpains are modulatory proteases that modify diverse cellular substrates and play essential roles in eukaryots. The best studied are animal cytosolic calpains. Here, we focus on enigmatic membrane-anchored calpains, their ... -
The nuclear GUCT domain-containing DEAD-box RNA helicases govern gametophytic and sporophytic development in Physcomitrium patens
Perroud, Pierre-francois; Demko, Viktor; Ako, Ako Eugene; Khanal, Rajendra; Bokor, Boris; Pavlovic, Andrej; Jasik, Jan; Johansen, Wenche (Peer reviewed; Journal article, 2021)Key message In Physcomitrium patens, PpRH1/PpRH2 are GUCT-domain-containing DEAD-BOX RNA helicases localize to the nucleus. They are implicated in cell and tissue development in all stages of the moss life cycle. Abstract ...