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dc.contributor.authorRahman, Mahfizur
dc.contributor.authorFalck-Ytter, Anne B.
dc.contributor.authorAntonsen, Øyvind G.
dc.contributor.authorStrætkvern, Knut O.
dc.identifier.citationRahman, M., Falck-Ytter, A. B., Antonsen, Ø. G. & Strætkvern, K. O. (2015). Reindeer lichen (Cladonia stellaris) from a Norwegian mountain region as a sustainable source of usnic acid. International Journal of Applied Research in Natural Products, 8(3), 17-23. Lokalisert på
dc.descriptionThis is an open access article. You can find it online by following this link: The article is published under a Creative Commons Attribution 3.0 Unported licens (CC BY 3.0) :
dc.description.abstractSummary. Usnic acid (UA) was extracted at 1.5-1.9 % dry weight from samples of Cladonia stellaris, a carpet-forming lichen growing abundantly in mountain areas in southeastern Norway. UA is known for its antibiotic activity as well as other bio-inhibitory functions, and is currently used in a number of formulations. Thus, the objective was to isolate and analyze UA to assess its availability. Preparations, made from lichen: acetone extraction (1:15) and from refining by recrystallization, were analyzed by one- and two-dimensional thin layer chromatography, gas chromatography, melting point, polarimetry and NMR. Only minor contaminants were observed, and both crude and refined preparations demonstrated properties (Rf-value, retention time, melting point and optical rotation) similar to the reference compound. However, polarimetry showed that C. stellaris contained the (-) enantiomer (>97 %) as opposed to Usnea-species where (+) UA is dominant. Both proton and 13C NMR confirmed structure identity to UA. Samples from four locations at different altitudes (250-650 m) around 62o north did not differ significantly (p< 0.05) in UA content. The study area has for a long time been carefully managed commercially for ornamental lichen harvesting to sustain regrowth. Thus applying the same practice, harvesting the lichen for recovering UA at high purity is highly feasible. Industrial relevance. Usnic acid has for a long time been subject to research for its biological activities, especially as a broad-sceptered antibiotic and more recently for its anti-cancerous effects in vitro. Considering the alarming spread of antibiotic resistance, UA can be a progressive alternative for those antibiotics, and to improve cancer treatment. Accumulating research data point to different biological effects of the two enantiomers. While the toxicity of the compound is still debated, UA is frequently used in health product formulations. The main commercial source Usnea barbata producing (+) UA is ubiquitous but limited to old-growth vegetation. C. stellaris remain a widespread, high-yielding producer of (-) UA in the boreal north, Fenno-scandinavia in particular. Under careful management, it can be efficiently harvested as already demonstrated in areas of Norway, and thereby become a valuable and sustainable source for large-scale production of UA.nb_NO
dc.publisherHealthy Synergies Publishers (USA)nb_NO
dc.rightsNavngivelse-DelPåSammeVilkår 3.0 Norge*
dc.subjectusnic acidnb_NO
dc.subjectacetone yieldnb_NO
dc.titleReindeer lichen (Cladonia stellaris) from a Norwegian mountain region as a sustainable source of usnic acidnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.subject.nsiVDP::Technology: 500::Biotechnology: 590nb_NO
dc.source.journalInternational Journal of Applied Research in Natural Productsnb_NO

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Navngivelse-DelPåSammeVilkår 3.0 Norge
Except where otherwise noted, this item's license is described as Navngivelse-DelPåSammeVilkår 3.0 Norge