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dc.contributor.authorRojek, Ewelina Katarzyna
dc.date.accessioned2019-04-11T08:27:31Z
dc.date.available2019-04-11T08:27:31Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/11250/2594154
dc.description.abstractCampylobacter jejuni and Campylobacter coli are zoonotic pathogens causing diarrhoeal illnesses. Between 2009 and 2017 campylobacteriosis affected a total of 26,676 people in Norway, mostly involving C. jejuni. Increased number of Campylobacter resistant to several antimicrobial drugs has become a public health concern, and further research into molecular mechanisms conferring resistance is needed. In this study, whole genome sequence (WGS) data from 35 C. jejuni and five C. coli isolates were used for: i) molecular typing using the core genome multilocus sequence typing (cgMLST) methodology; ii) conduct evolutionary relationships analysis between isolates based on core genome and single nucleotide polymorphisms; iii) identification of antimicrobial resistance genotypes, to correlate to resistance phenotypes; iv) identification of cluster thresholds for outbreak detection. The higher discriminatory power of WGS revealed that two isolates which had been phenotypically classified as C. jejuni were, in fact, C. coli. Moreover, phylogenetic analysis enabled to separate the epidemiologically related strains from those which were non-related, resulting in the identification of three clusters. Reference-based cgMLST approach with 637 shared loci established that epidemiologically linked isolates within an outbreak differed by ≤ 2 allelic variations. A similar number of genomic differences (≤ 2 SNPs) was observed by SNP-based phylogeny. Antimicrobial susceptibility testing followed by the detection of resistance genotypes revealed that 26/40 isolates were resistant to at least one antimicrobial drug and a total of 9 isolates were multidrug resistant. Mostly, antimicrobial resistance was linked to resistance genes such as oxa-61, homologs of tet and aph, and genes encoding cmeABC efflux pump system; however phenotypic resistance towards erythromycin, tetracycline, and gentamycin was detected within isolates that did not carry cme, tet, or aph genes homologs. This study shows that WGS, could be used for phylogenetic inference using tools such as multilocus sequence typing; WGS is also useful for investigation of bacterial outbreaks and provides high resolution for purposes of surveillance, prevention, and control of bacterial illnesses.nb_NO
dc.language.isoengnb_NO
dc.titleMolecular typing, antimicrobial resistance profiling and phylogeny of Campylobacter based on whole genome sequencingnb_NO
dc.typeMaster thesisnb_NO


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