• norsk
    • English
  • English 
    • norsk
    • English
  • Login
View Item 
  •   Home
  • Master- og hovedoppgaver / Master thesis
  • Master i næringsrettet bioteknologi / Master of applied and commercial biotechnology M2NRBIOTEK
  • View Item
  •   Home
  • Master- og hovedoppgaver / Master thesis
  • Master i næringsrettet bioteknologi / Master of applied and commercial biotechnology M2NRBIOTEK
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Population Genetic Analysis of Red Foxes (Vulpes vulpes) in Hedmark Country, Norway - A Pilot Study

Manivannan, Aarathi
Master thesis
Thumbnail
View/Open
Manivannan.pdf (3.521Mb)
URI
http://hdl.handle.net/11250/132361
Date
2013
Metadata
Show full item record
Collections
  • Master i næringsrettet bioteknologi / Master of applied and commercial biotechnology M2NRBIOTEK [96]
Abstract
Ecological and environmental studies commonly depended on the knowledge of genetic

variation among individuals within a population and most of the population studies were

carried out using genetic markers. In this thesis, individuals of Vulpes vulpes species were

genotyped using microsatellite markers to study their population diversity. DNAs were

isolated successfully from the muscle tissues of 33 individuals and in some cases, from the

hair and scat of the foxes. Twenty microsatellite primers with 1 sex chromosome marker

were successfully designed using MP primer downloaded program out of which 16 primers

amplified the DNA template samples successfully. The Amplicons were successfully

genotyped and checked for genotyping errors. The LOSITAN program identified two of the

markers to be under selection and the Micro-Checker program adjusted the alleles, which

were identified as ‘null’ alleles. The analysis was performed on three different types of data:

original genotype data, adjusted genotype data with the null allele markers and genotype

data without the null allele markers. The marker under balanced selection was also added to

verify its effect on the study. The STRUCTURE program assembled the individuals into

different clusters. The Arlequin program estimated that the individuals grouped using

original genotype data proved genetically variable, whereas individuals grouped using

adjusted null alleles did not show any significant variance. Original genotype data looks

promising as the loci were not significantly in Linkage Disequilibrium but this can be

justified by increasing the number of individuals for the study. Analysis using genotypic

data without null allele markers did not vary from the analysis using original genotypic

data. Addition of marker under balanced selection showed significant Linkage

disequilibrium in the study of all the three types of data. Relationship between the

individuals showed that many individuals were half- siblings and 4 of them were full

siblings.
Description
Mastergradsoppgave i næringsrettet bioteknologi, Avdeling for lærerutdanning og naturvitenskap, Høgskolen i Hedmark, 2013. Master of applied and commercial biotechnology.

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit
 

 

Browse

ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDocument TypesJournalsThis CollectionBy Issue DateAuthorsTitlesSubjectsDocument TypesJournals

My Account

Login

Statistics

View Usage Statistics

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit