• norsk
    • English
  • English 
    • norsk
    • English
  • Login
View Item 
  •   Home
  • Artikler, rapporter, filmer / Articles, reports, movies
  • Artikkel - fagfellevurdert vitenskapelig / Articles - peer-reviewed
  • View Item
  •   Home
  • Artikler, rapporter, filmer / Articles, reports, movies
  • Artikkel - fagfellevurdert vitenskapelig / Articles - peer-reviewed
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Life without Oxygen: Gene Regulatory Responses of the Crucian Carp (Carassius carassius) Heart Subjected to Chronic Anoxia

Stensløkken, Kåre-Olav; Ellefsen, Stian; Vasieva, Olga; Fang, Yongxiang; Farrell, Anthony P.; Olohan, Lisa; Vaage, Jarle; Nilsson, Göran E.; Cossins, Andrew R.
Journal article, Peer reviewed
Thumbnail
View/Open
Ellefsen 2014.pdf (1005.Kb)
URI
http://hdl.handle.net/11250/275535
Date
2014
Metadata
Show full item record
Collections
  • Artikkel - fagfellevurdert vitenskapelig / Articles - peer-reviewed [1246]
Original version
10.1371/journal.pone.0109978
Abstract
Crucian carp are unusual among vertebrates in surviving extended periods in the complete absence of molecular oxygen.

During this time cardiac output is maintained though these mechanisms are not well understood. Using a high-density

cDNA microarray, we have defined the genome-wide gene expression responses of cardiac tissue after exposing the fish at

two temperatures (8 and 13uC) to one and seven days of anoxia, followed by seven days after restoration to normoxia. At

8uC, using a false discovery rate of 5%, neither anoxia nor re-oxygenation elicited appreciable changes in gene expression.

By contrast, at 13uC, 777 unique genes responded strongly. Up-regulated genes included those involved in protein turnover,

the pentose phosphate pathway and cell morphogenesis while down-regulated gene categories included RNA splicing and

transcription. Most genes were affected between one and seven days of anoxia, indicating gene regulation over the

medium term but with few early response genes. Re-oxygenation for 7 days was sufficient to completely reverse these

responses. Glycolysis displayed more complex responses with anoxia up-regulated transcripts for the key regulatory

enzymes, hexokinase and phosphofructokinase, but with down-regulation of most of the non-regulatory genes. This

complex pattern of responses in genomic transcription patterns indicates divergent cardiac responses to anoxia, with the

transcriptionally driven reprogramming of cardiac function seen at 13uC being largely completed at 8uC.
Journal
PLoS ONE

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit
 

 

Browse

ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDocument TypesJournalsThis CollectionBy Issue DateAuthorsTitlesSubjectsDocument TypesJournals

My Account

Login

Statistics

View Usage Statistics

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit