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dc.contributor.authorNarud, Birgitte
dc.contributor.authorKhezri, Abdolrahman
dc.contributor.authorZeremichael, Teklu Tewoldebrhan
dc.contributor.authorStenseth, Else-Berit
dc.contributor.authorHeringstad, Bjørg
dc.contributor.authorJohannisson, Anders
dc.contributor.authorMorrell, Jane
dc.contributor.authorCollas, Philippe
dc.contributor.authorMyromslien, Frøydis Deinboll
dc.contributor.authorKommisrud, Elisabeth
dc.date.accessioned2021-11-12T14:23:24Z
dc.date.available2021-11-12T14:23:24Z
dc.date.created2021-02-26T13:42:09Z
dc.date.issued2021
dc.identifier.citationMolecular Reproduction and Development. 2021, 88 (3), 187-200.en_US
dc.identifier.issn1040-452X
dc.identifier.urihttps://hdl.handle.net/11250/2829400
dc.description.abstractn this study, the complexity of chromatin integrity was investigated in frozen-thawed semen samples from 37 sires with contrasting fertility, expressed as 56-day non-return rates (NR56). Protamine deficiency, thiols, and disulfide bonds were assessed and compared with previously published data for DNA fragmentation index (DFI) and high DNA stainability (HDS). In addition, in vitro embryo development and sperm DNA methylation were assessed using semen samples from 16 of these bulls. The percentages of DFI and HDS were negatively associated with NR56 and cleavage rate and positively associated with sperm protamine deficiency (p < 0.05). Significant differences in cleavage and blastocyst rates were observed between bulls of high and low NR56. However, once fertilization occurred, further development into blastocysts was not associated with NR56. The differential methylation analysis showed that spermatozoa from bulls of low NR56 were hypermethylated compared to bulls of high NR56. Pathway analysis showed that genes annotated to differentially methylated cytosines could participate in different biological pathways and have important biological roles related to bull fertility. In conclusion, sperm cells from Norwegian Red bulls of inferior fertility have less compact chromatin structure, higher levels of DNA damage, and are hypermethylated compared with bulls of superior fertility.en_US
dc.language.isoengen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleSperm chromatin integrity and DNA methylation in Norwegian Red bulls of contrasting fertilityen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber187-200en_US
dc.source.volume88en_US
dc.source.journalMolecular Reproduction and Developmenten_US
dc.source.issue3en_US
dc.identifier.doihttps://doi.org/10.1002/mrd.23461
dc.identifier.cristin1894059
dc.relation.projectNorges forskningsråd: 268048en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal